Carbapenem-resistant GES-5-producing Klebsiella pneumoniae in Southern Brazil.

GES-type enzymes belong to Ambler Class A plasmidencoded -lactamases. Although GES enzymes usually exhibit extended-spectrum -lactamases (ESBL) properties, changes in its active site enhance its activity conferring the ability to hydrolyze carbapenems.1 GES-type carbapenemases (GES2, GES-4, GES-5, GES-8, GES-11, GES-18, GES-20) have already been described in several countries.2 Although it has been reported in a Klebsiella pneumoniae from Brazil in 2010, in a patient admitted in 2008,3 no other isolate has ever since been reported in this country. Here, we described another carbapenem-resistant K. pneumoniae (CRKP) isolate carrying the blaGES-5 gene in Brazil. The CRKP, identified by VITEK2 system (bioMeriéux, France), was recovered from the blood of an adult patient admitted to a university hospital in Porto Alegre, Brazil, in 2011. The patient had acute myeloid leukemia with recent exposure to many antibiotics including piperacillin–tazobactam, polymyxin B, meropenem, vancomycin, and cefepime. Despite treatment with polymyxin B and tigecycline, the patient ultimately died six days after CRKP recovering. Minimum inhibitory concentrations (MICs), performed by Etest®, and the phenotypic tests for carbapenemases detection are shown in Table 1. The presence of blaGES was demonstrated by multiplex real-time PCR that also included blaKPC, blaVIM, blaNDM, blaOXA-48 and blaIMP genes.4 Sequencing of blaGES gene was performed using the primers previously described.5 GenBank was used to access the GES sequences deposited to date and BioEdit program was used to compare the similarity between sequences, and revealed the presence of a GES-5. Plasmidial DNA from CRKP was obtained from alkaline lysis and it was electroporated into an Escherichia coli Top10 (Invitrogen). Transformants were selected on Luria-Bertani agar containing 0.5 g/mL of ceftazidime. The plasmid electroporation resulted in transformants, which confirmed the presence of blaGES by PCR (Table 1). We reported the second case of a CRKP carrying the blaGES-5 gene in Brazil. Although the first GES-5-producing CRKP was reported in São Paulo in 2008,3 there has been no other report of this enzyme in Enterobacteriaceae since then in Brazil. Table 1 – Phenotypic characterization of Klebsiella pneumoniae and Escherichia coli transformant carrying blaGES-5. MIC ( g/mL)a